1. Title- Zebra Fish as an Alternative Model for Determining the Median Lethal Concentration of Cobra Venom
Team: PI Investigator: Dr. Mrunal Ghag Sawant Co-Investigator: Mrs. Swati U. Mumbarkar Scientific Assistant: Mrs. Meenal Mulkutkar
M.Sc. Student: Ghatkar Madhura Pramod Prerna
Funding Agency: Academic Research / Department of Toxicology Duration: 15th January 2023 to 14th January 2026
Abstract: This proposal is focused towards validation and standardization of assays in lower sentinel animals like zebrafish. The aim of the study is the standardization and validation of the Median Lethal Concentration and Lethal Concentration of Cobra venom range in Zebrafish.
2. Title- Determining genotoxic potential of Cobra Venom using in vivo method
Team: PI Investigator: Dr. Mrunal Ghag Sawant Co-Investigator: Mrs. Swati U. Mumbarkar Scientific Assistant: Mrs. Meenal Mulkutkar
M.Sc. Student: Ms. Shreya Thakur
Funding Agency: Academic Research/ Department of Toxicology Duration: 15th January 2023 to 14th January 2026
Abstract: The Indian Cobra’s venom mainly consists of a powerful post-synaptic neurotoxin and cardiotoxin. Present work will focus on exploring the effects of snake venom on the genetic material/molecular level using rodents.
3. Title- Quantitative estimation of nitric oxide and glutathione in cobra venom-induced oxidative stress in zebrafish embryos
Team: PI Investigator: Dr. Mrunal Ghag Sawant Co-Investigator: Mrs. Swati U. Mumbarkar Scientific Assistant: Mrs. Meenal Mulkutkar
M.Sc. STRIP Student: Ms. Rutuja Shikhare
Funding Agency: Haffkine Institute Duration: January 2023 to June 2023
Abstract: In this study, the oxidative stress and antioxidant defence caused due to Cobra venom was studied, for which the concentration of nitric oxide, an oxidant, and the concentration of GSH, an antioxidant were measured.
4. Title- Design, synthesis and evaluation of substituted Indole and quinoline derivatives as a potent tyrosine kinase inhibitor {N”(3,5 dihydrobenylidene)-6-methoxy-2-(4-methoxyphenyl) quinolone -4- carbohydrazide: (4a) MW: 443.46 Molecular Formula : C25H21N3O5}
Team: PI Investigator: Dr. Mrunal Ghag Sawant Co- PI : Dr. Ashish Asronkar Co-Investigator: Mrs. Swati U. Mumbarkar Scientific Assistant: Mrs. Meenal Mulkutkar M.Sc. Strip Student: Mr. Tanmay Shinde
Funding Agency: Haffkine Institute Duration: 6 months (December 2023 to May 2024)
Abstract: Aiming to extend pursue our fascination in the synthesis of novel quinolone derivatives with potential biological activity, we designed new quinoline derivatives that included Schiff base, pyrazole moieties. The quinoline compounds that emerged were synthesized and assessed against triple negative breast cancer cells. The majority of the compounds demonstrated anti-cancer action. Among them, quinoline heterocyclic ligands demonstrated an excellent inhibitory ratio, which is corroborated by docking studies with the highest binding affinity and lowest docking score. In the current study, we are using the NCE ligands to study acute intravenous toxicity in Swiss albino mice. Based on the results of the present study, it is concluded that the N”(3,5 dihydrobenylidene)-6-methoxy-2-(4-methoxyphenyl) quinolone -4- carbohydrazide: (4a) is non-toxic by Intravenous route and is classified under GHS category 4 / Unclassified.
5. Title- Design, synthesis and evaluation of substituted Indole and quinoline derivatives as a potent tyrosine kinase inhibitor {N”(3, 4-chlorobenylidene)-6-methoxy-2-(4-methoxyphenyl) quinolone-4- carbohydrazide: (4b) MW: 445.90 Molecular Formula : C25H20CIN3O5}
Team: PI Investigator: Dr. Mrunal Ghag Sawant Co- PI : Dr. Ashish Asronkar Co-Investigator: Mrs. Swati U. Mumbarkar Scientific Assistant: Mrs. Meenal Mulkutkar. M.Sc. STRIP Student: Miss Siddhi Nigade
Funding Agency: Haffkine Institute
Abstract: Aiming to extend pursue our fascination in the synthesis of novel quinolone derivatives with potential biological activity, we designed new quinoline derivatives that included Schiff base, pyrazole moieties. The quinoline compounds that emerged were synthesized and assessed against triple negative breast cancer cells. The majority of the compounds demonstrated anti-cancer action. Among them, quinoline heterocyclic ligands demonstrated an excellent inhibitory ratio, which is corroborated by docking studies with the highest binding affinity and lowest docking score. In the current study, we are using the NCE ligands to study acute intravenous toxicity in Swiss albino mice. Based on the results of the present study, it is concluded that the N”(3, 4-chlorobenylidene)-6-methoxy-2-(4-methoxyphenyl)quinolone-4- carbohydrazide: (4b) is non-toxic by Intravenous route and is classified under GHS category 4 / Unclassified.
6. Title- To Study the interplay of oxidative Stress, Tissue Damage and Immunological Effects of Viperidae Venom in Mice
Team: Principal Investigator and Ph.D. Student - Dr. Mrunal Ghag Sawant/ Guide/Mentor - Dr. Sandeepan Mukherjee
Funding Agency: Haffkine Institute
Duration: 5 Year (Registration date: 05/05/2022)
Status: Ongoing
Abstract: Snakebite envenomation is a serious medical problem in many developing tropical and subtropical countries and is categorized as a neglected tropical disease due to critical shortages in the production of antivenom. To effectively treat snakebites and to understand the need of species specific antivenom, it is important to determine the lethal potency and pathophysiological effects induced by specific snake venoms. In the current study, we assess and compare the lethality of Viperidae venoms that are the primary causes of severe local tissue necrosis.
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